Fifteen CRAB isolates were collected from patientsadmitted to Phyathai II International Hospital, Bangkok, Thailand during August 2014– April 2015. Polymerase chain reaction (PCR) amplification and DNA sequencing wereused to identify blaOXA23, blaOXA40, blaOXA48, blaOXA58, blaIMP, blaVIM, blaKPC, and blaNDM.Clonal relationships were explored by using repetitive element palindromic (REP)-PCR.
The CRAB isolates were categorized by REP-PCR in 8 groups [A-H], with53.3% belonging to group A, whereas the remaining 7 clones were in each member ofB-H, respectively. The blaOXA23 was detected in most CRAB isolates (86.7%) whereas onlytwo isolates harbored blaNDM1 with blaOXA23 (13.3%).
Most CRAB strains carried blaOXA23 as reported in several related studies but our finding confirmed the emergence of CRAB carrying multiple types of carbapenemase genes in Thailand. This is a worrying phenomenon that concerns the spread of such CRAB genotypes.
OXA carbapenemase, carbapenemase, metallo-beta lactamase, clonal relationship
Received: July 30, 2018
Revision received: November 05, 2018
Accepted after revision: April 23, 2019
BKK Med J 2019;15(2): 149-153.