Electronic ISSN 2287-0237

VOLUME

ACUTE FULMINANT HEPATITIS DUE TO HERPES SIMPLEX TYPE 1 INFECTION

FEBRUARY 2014 - VOL.7 | CASE REPORT
Keywords:

fulminant hepatitis, herpes simplex type 1, herpes simplex virus, HSV

MEDIA
Table 1:
Results of blood chemistry and transaminase.
Table 2:
Initial results of viral and other etiologic tests that may be a cause of fulminant hepatitis.
Table 3:
The Ct value from consecutive samples show that the viral load from the first serum collected is high, as the Ct value is dependent on the amount of target present at the beginning of the reaction. The signal from the HSV on 12/10/2013 is a signal from the primer dimer. The Tm of HSV1 PCR product in oC from consecutive samples is as follows: HSV on 4/9/2013 to HSV on 28/9/2013 and a Tm of primer dimer HSV on 12/10/2013.
Figure 1:
The amplification plot of real-time SYBR Green I PCR assay for Herpes Simplex type 1 from consecutive serum samples from a fulminant hepatitis patient. (The light green is the sample from 4/09/2013, the red from 6/09/2013, the purple from 9/09/2013, the pink from 14/09/2013, the blue from 20/09/2013, the yellow from 28/09/2013 and the green from 12/10/2013).
Figure 2:
The melting curve profiles of HSV1 real-time PCR. The HSV1 PCR products have a Tm around 87.8-88.0 but the primer dimer product has a Tm of around 85.7 which is clearly separate from the HSV1 PCR product.
Figure 3:
Liver pathology of HSV hepatitis: Zones of hepatocyte necrosis surrounded by hemorrhage without significant inflammation [100x magnification; Hema- toxylin-Phloxine-Saffron (HPS) stain].
Figure 4A-B:
HSV inclusions in infected hepatocyte. (A) Viral inclusions are readily visible in infection hepatocytes. (600x magnification; Hematoxylin-Phloxine-Saffron (HPS) stain). (B) Immunohistochemistry for HSV virus highlights viral inclusions (600x magnification).
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